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货号：C3010-0500
规格：500ml
产品名称：RPMI 1640 With L-Glutamine
【Product Description】
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
| Ingredients | Mg/L |
| INORGANIC SALTS | |
| Calcium chloride dihydrate | 100.000 |
|
Magnesium sulphate anhydrous |
48.840 |
| Potassium chloride |
400.000 |
| Sodium chloride | 6000.000 |
| Sodium dihydrogen phosphate anhydrous | 800.000 |
|
|
|
| AMINO ACIDS | |
| Glycine | 10.000 |
|
L-Arginine hydrochloride |
241.000 |
|
L-Asparagine |
50.000 |
|
L-Aspartic acid |
20.000 |
|
L-Cystine dihydrochloride |
65.200 |
| L-Glutamic acid |
20.000 |
| L-Glutamine | 300.000 |
| L-Histidine hydrochloride monohydrate | 20.960 |
| L Hydroxyproline |
20.000 |
| L-Isoleucine | 50.000 |
| L-Leucine |
50.000 |
| L-Lysine hydrochloride | 40.000 |
| L-Methionine | 15.000 |
| L-Phenylalanine |
15.000 |
| L-Proline |
20.000 |
| L-Serine |
30.000 |
| L-Threonine |
20.000 |
| L-Tryptophan |
5.000 |
| L-Tyrosine Disodium Salt |
28.830 |
| L-Valine |
20.000 |
|
|
|
| VITAMINS | |
| Choline chloride |
3.000 |
| D-Biotin |
0.200 |
| D-Ca Pantothenate | 0.250 |
| Folic acid |
1.000 |
| Niacinamide |
1.000 |
| Pyridoxal hydrochloride |
1.000 |
| Riboflavin |
0.200 |
| Thiamine hydrochloride | 1.000 |
| Vitamin B12 | 0.005 |
| i-Inositol |
35.000 |
|
p-Amino benzoic acid (PABA) |
1.000 |
|
|
|
| OTHERS | |
|
D-Glucose |
2000.000 |
|
Glutathione reduced |
1.000 |
| Phenol red sodium salt | 5.300 |
| Sodium bicarbonate |
2000.000 |
【Storage and Stability】
RPMI 1640 , with L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments ;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
RPMI 1640, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
