品牌：
货号：
规格：
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货期：
货号：C3134-0500
规格：500ml
产品名称：F-12 Nutrient Mixture (Ham's), With L-Glutamine
【Product Description】
Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured.
Ham's Nutrient Mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L cells. It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells. This medium is also the medium of choice for clonal toxicity assay using CHO cells.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
| Ingredients | Mg/L |
| INORGANIC SALTS | |
| Calcium chloride dihydrate | 44.100 |
| Copper sulphate pentahydrate |
0.0025 |
| Ferric nitrate nonahydrate |
0.834 |
|
Magnesium sulphate anhydrous |
57.650 |
| Potassium chloride |
223.600 |
| Sodium chloride | 7599.000 |
| Sodium bicarbonate |
1176.000 |
| Sodium dihydrogen phosphate anhydrous | 142.040 |
| Zinc sulphate tetrahydrate |
0.863 |
|
|
|
| AMINO ACIDS | |
| Glycine | 7.500 |
| L-Alanine |
8.910 |
|
L-Arginine hydrochloride |
210.700 |
| L-Asparagine anhydrous |
15.010 |
| L-Aspartic acid |
13.300 |
|
L-Cystine dihydrochloride |
35.120 |
| L-Glutamic acid |
14.700 |
| L-Glutamine | 146.000 |
| L-Histidine hydrochloride monohydrate | 22.960 |
| L-Isoleucine | 3.940 |
| L-Leucine |
13.100 |
| L-Lysine hydrochloride | 36.500 |
| L-Methionine | 4.480 |
| L-Phenylalanine |
4.960 |
| L-Serine |
10.500 |
| L-Threonine |
11.900 |
| L-Tryptophan | 2.040 |
| L-Tyrosine Disodium Salt |
7.810 |
| L-Valine |
11.700 |
|
|
|
| VITAMINS | |
| Biotin |
0.0073 |
| Choline chloride |
13.960 |
| D-Ca Pantothenate | 0.480 |
| Folic acid | 1.320 |
| Nicotinamide |
0.037 |
| Pyridoxal hydrochloride |
0.062 |
| Riboflavin |
0.038 |
| Thiamine hydrochloride | 0.340 |
| Vitamin B12 |
1.360 |
| i-Inositol |
18.000 |
|
|
|
| OTHERS | |
|
D-Glucose |
1801.600 |
| Hypoxanthine Sodium Salt | 4.770 |
| Linoleic acid | 0.084 |
| Lipoic acid | 0.210 |
| Phenol red Sodium Salt | 1.240 |
| Putrescine dihydrochloride | 0.161 |
| Sodium pyruvate | 110.100 |
| Thymidine | 0.730 |
【Storage and Stability】
Nutrient Mixture F-12 Ham, With L-Glutamine should be kept 2 8°C. The product is light sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/ flocculent debris/ granulation/ particulates precipitates or sediments;
(c). Insolubility;
(d). And/or decrease in expected perf ormance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2 8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
Nutrient Mixture F-12 Ham, With L-Glutamine is tested for sterility , pH, O smolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
