名称：BIOSILK 521

用途：An Optimal 3d Culture Environment for Expansion and Differentiation of Human PSCs.

货号：BS521-0101

保存温度：-80℃

Biosilk 521 is a natural biomaterial made of recombinant spider silk protein, functionalized with human recombinant laminin 521 protein (Biolaminin 521), designed for 3D cell culture. Laminin 521 is a key cell adhesion protein of the natural stem cell niche, providing the Biosilk 521 material with unique, functional properties that are ideal for integration, proliferation and subsequent lineage-specific differentiation of human pluripotent stem cells in a 3D format.

A NATURAL BIOMATERIAL DESIGNED FOR 3D CELL CULTURE OF HUMAN PLURIPOTENT STEM CELLS 

Biosilk 521 is a natural biomaterial made of recombinant spider silk protein, functionalized with human recombinant laminin 521 protein (Biolaminin 521), designed for 3D cell culture. Laminin 521 is a key cell adhesion protein of the natural stem cell niche, providing the Biosilk 521 material with unique, functional properties that are ideal for integration, proliferation and subsequent lineage-specific differentiation of human pluripotent stem cells in a 3D format.

Biosilk 521 is a biocompatible, biodegradable and non-immunogenic biomaterial, which facilitates the use in clinical applications. The extendable Biosilk 521 macrofibre material can self-assemble into several formats; foams, fiber bundles, and thin films. 

A MORE BIOLOGICALLY RELEVANT CULTURE ENVIRONMENT

Compared to 2D cultures, a 3D culture is a more biologically relevant culture environment with a higher degree of architectural complexity that retains homeostasis for longer.

Contrary to many other 3D scaffolds, the cells can be added to the Biosilk 521 solution before the network stabilizes, resulting in a uniform integration of the cells between the microfibers. Unlike cells cultured in a hydrogel, cells seeded in the Biosilk scaffold attach along the microfibers and are in contact with other cells like in tissue. Moreover, the cells proliferate more efficiently and migrate in the Biosilk 521 scaffold and have full and even access to both the medium, matrix, and neighboring cells, compared to when encapsulated in a hydrogel.

FEATURES AND SPECIFICATIONS:

• Flexible 3D culture system that can be assembled into different formats

• Elastic microfibers mimicking the ECM

• Biologically relevant hPSC culture environment

• Biocompatible

• Biodegradable

• Non-immunogenic

• Defined and animal origin-free to the primary level

• For research use only

BIOSILK 521 FOAM MORPHOLOGY

Representative pictures of the Biosilk 521 foam taken from above day 0, 1 and 3 after cell seeding. The initial small bubbles merge into bigger bubbles that disperse after a few days, resulting in a 3D scaffold with integrated cells. 

CELLS ATTACHED TO B10SILK 521 

Micrographs of human ES cells attached to Biosilk 521 days 2, 3 and 5 after seeding. When the bubbles have dispersed, the Biosilk scaffold with cells organized between the microfibers can be visualized. 

EFFICIENT PROLIFERATION WITH MAINTAINED STEMNESS MARKER EXPRESSION

Human ES cells that are cultured in Biosilk 521 in media for culture of pluripotent cells efficiently integrate, proliferate and form colonies along the microfibres with a maintained expression of sternness marker (NANOG; red). Scale bar 50 µm. 

Human ES cells (HS980) and iPS cells (iPSC3) seeded at 50.000 cells/foam were cultured for 4 days in three different pluripotent cell culture media. The cells were detached with TrypLE solution and cell amount and viability was measured. 

PLURIPOTENT CELLS CAN DIFFERENTIATE INTO THREE LINEAGES 

Human pluripotent stem cells were cultured in the Biosilk 521 scaffold for 2-3 days before lineage-specific differentiation to ectoderm (7 days, NESTIN and PAX6), endoderm (3 days, FOXA2) and mesoderm (12 days, NKX2.5). DAPI was used as a nuclear counterstain. 

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Technical Resources

Application note

Instruction For Use 011

Technical Guide Video

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