产品简述

重组CHO细胞分泌的六聚体蛋白hexeramized lgG1在平盘中不容易得到。如在T150 Flask培养，只能得到60%的六聚体，而大约有 40%的蛋白无法正确折叠，而呈四聚体的构型。

而在FiberCell系统中大约有95%的蛋白以正确折叠的六聚体（由6个带有3个IgA尾的lgG1亚基组成）形式存在的，几乎不表达四聚体。 总体枳4L的体系10周可以生产475mg蛋白。

150cm2Flask（上图）与FiberCell系统生产（下图）人重组IgG六聚体比较

峰 A：T150 Flask中培养，得到60%的六聚体

峰 B：T150 Flask中培养，得到40%的四聚体

峰 C：FiberCell中培养，得到95%的六聚体

采用Fibercell系统生产重组蛋白的优点：

• 每天可收获重组蛋白1-10mg

• 蛋白浓度提高100倍

• 降低血清需求利于纯化

• 高浓度小体积，后续处理简单

• 优化的细胞培养条件可提高蛋白质组装、折叠与糖基化

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