产品简述

杂交瘤细胞会分泌转化生长因子-β（TGF-β），它是一种会抑制杂交瘤细胞生长的细胞因子。功能型TGF-β的分子量大约是 27kD，截留分子量大的培养筒（Cartridge）允许TGF- β扩散通过，所以由于浓度差异，TGF-β会自然进入循环培养基，可以减小或者消除对细胞的抑制效果；而杂交瘤细胞分泌的抗体则在ECS（中空纤维外空间）堆枳， 并可达到很高的浓度。

细胞附着在多孔支架上，类似于体内生长方式。在中空纤维系统内，细胞可以维持活力和分泌代谢物数个月或以上。中空纤维系统内的细胞不需要传代，因为一定比例的细胞会处于静止期，即它们会分泌蛋白但停止分裂。例如，有在Fibercell 系统内培养杂交瘤细胞生产单抗一年以上，另外有连续培养胶质瘤细胞近两年。

     产物未经纯化的电泳图

MAb 1：体积60ml，浓度2.8mg/ml，共168mg，3周消耗9L培养基

MAb 2：体积70ml，浓度2.3mg/ml，共159mg，3周消耗11L培养基

采用Fibercell系统生产单克隆抗体的优点：

• #C2011每2天可生产单抗10-50mg，消耗1L培养基

• 单次收获体积15-20ml，抗体浓度：0.5-5mg/ml

• 内毒素负荷低于腹水培养10倍

• 易于适应血清替代物（CDM-HD）及无血清培养

• 容易得到嵌合的人源化和其他非鼠源单抗

参考文献

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