VitroGel 3D成功应用案例-TheWell 3D水凝胶-细胞生物学-产品中心-上海昊则生物科技有限公司

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VitroGel 3D为细胞创造一个功能性和优化的环境，让细胞感觉在家一样。从2D包被，3D细胞培养到动物注射，VitroGel 3D让利用相同系统进行体内体外研究成为可能。用户使用VitroGel 3D系统主要用于研究癌症和肿瘤细胞生物学，干细胞和组织工程，药物发现和毒性研究等方面。

下表列出了已在VitroGel 3D系统中成功测试的细胞系。可根据不同的细胞系和培养基组分优化VitroGel 3D水凝胶系统，以得到更好的结果。推荐培养不同的细胞系时，都进行水凝胶浓度梯度测试。

Tables of successful cell types with Vitrogel 3D (TWG001)

CELL TYPES	APPLICATIONS	CULTURE MEDIUM	DILUTION
4T1 cells	3D culture	RPMI 1640 with 10% FBS	1:2
BL5 human beta cells	2D and 3D culture	DMEM with 10%FBS	1:3
CD8+ T cells	3D culture	RMPI 1640 with 10% FBS	1:3
HeLa cells	3D culture	DMEM with 10%FBS	1:3
Human Nthy-ori 3-1 cells	3D culture	RPMI 1640 with 10% FBS	1:3
KHOS cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
MDA-MB-231 cells	3D culture	RPMI 1640 with 10% FBS	1:2
Priess human lymphoblastoid cells	2D and 3D culture	RPMI 1640 with 10% FBS	1:3
Red Blood Cells	3D culture	Alsever's solution	1:1 to 1:3
T47D cells	3D culture	RPMI 1640 with 10% FBS	1:2
U2OS cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3

CELL TYPES	APPLICATIONS	CULTURE MEDIUM	DILUTION
4T1 cells	3D culture	RPMI 1640 with 10% FBS	1:2
A549 cells	3D culture	DMEM with 10% FBS	1:1 to 1:3
Au565 cells	3D culture	RPMI 1640 with 10% FBS	1:3
Beta TC3 cells	3D culture	DMEM with 10% FBS	1:3
BT 474 cells	3D culture	DMEM with 10% FBS	1:3
DLD1 cells	3D culture	DMEM with 10% FBS	1:1 to 1:3
Fuji cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
H460 cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
HCT 116 cells	2D and 3D culture	McCoys 5 with 10% FBS	1:1
HCT-8 cells	3D culture	RPMI 1640 with 10% FBS	1:3
HEK 293 cells	3D culture	DMEM with 10% FBS	1:3
Hela cells	2D and 3D culture	DMEM with 10% FBS	1:3
HepG2 cells	3D culture	DMEM with 10% FBS	1:1 to 1:3
Human iPSCs	2D and 3D culture	mTeSR1	1:3
Ins-1 Cells	3D culture	RPMI 1640 with 10% FBS	1:3
MCF-12A cells	3D culture	DMEM/F-12 with 10% FBS	1:3
MDA-MB-231 cells	3D culture	L-15 medium with 10% FBS	1:1 to 1:3
Melanoma cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
OVCAR-3 cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
Panc-1 cells	3D culture	DMEM with 10% FBS	1:1 to 1:3
SKB3 cells	2D and 3D culture	DMEM with 10% FBS	1:1 to 1:3
SYO-1 cells	3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
T47D cells	3D culture	RPMI 1640 with 10% FBS	1:3
UD 145 cells	3D culture	EMEM with 10% FBS	1:1 to 1:3

CELL TYPES	APPLICATIONS	CULTURE MEDIUM	DILUTION
MCF-7 cells	2D and 3D culture	DMEM with 10% FBS	1:1 to 1:3
MCF-12A cells	3D culture	DMEM/F-12 with 10% FBS	1:3
MDA-MB-231 cells	3D culture	L-15 medium with 10% FBS	1:1 to 1:3
OP9 cells	2D and 3D culture	Alpha-MEM with 10% FBS	1:3
Panc-1 cells	2D and 3D culture	DMEM with 10% FBS	1:1 to 1:3
SF 268 cells	2D and 3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
SF 295 cells	2D and 3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
SNB 75 cells	2D and 3D culture	RPMI 1640 with 10% FBS	1:1 to 1:3
U-87 MG cells	2D and 3D culture	EMEM with 10% FBS	1:1 to 1:3
U-251 MG cells	2D and 3D culture	EMEM with 10% FBS	1:1 to 1:3